What is the purpose of the EMB agar?

EMB agar is used in water quality tests to distinguish coliforms and fecal coliforms that signal possible pathogenic microorganism contamination in water samples.

What is the purpose of EMB agar quizlet?

EMB agar is used to stain gram negative bacteria. It is used to isolate fecal coliforms(G- bacteria rod)*** . It’s used to distinguish between lactose fermenting coliforms and lactose non fermenting coliforms.

What is an EMB plate and how what is it specifically selective and differential for?

Eosin methylene blue (EMB, also known as “Levine’s formulation”) is a selective stain for Gram-negative bacteria. EMB contains dyes that are toxic to Gram-positive bacteria. EMB is the selective and differential medium for coliforms. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1.

What is the purpose of eosin methylene blue agar quizlet?

What are the uses? -EMB agar is used to isolate Gram negatives from clinical samples and to subsequently differentiate between lactose-fermenting coliforms and lactose-nonfermenting noncoliforms. -Eosin Methylene Blue (EMB) is used for the isolation of fecal coliforms.

What bacteria grows on EMB?

Colony Morphology

Organism	Colonial appearance on EMB agar
Enterobacter aerogenes	Colonies are 4-6mm in diameter, raised and mucoid, tending to become confluent. No metallic sheen, grey-brown centers by transmitted light
Salmonella and Shigella spp	Translucent and colorless colonies
Pseudomonas spp	Colorless irregular colonies

What is the purpose of peptone in EMB agar?

What does Peptone do in Eosin Methylene Blue (EMB) Agar? It contains a mixture of carbon, nitrogen, and other nutritional components. Sugars are included to encourage growth of enteric bacteria and used to differentiate the bacteria based on their color reactions created when combined with the dyes.

What are the two purposes of the dye in EMB agar?

Eosin Y and methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram positive organisms.

What is the principle of EMB?

Principle of EMB Agar Gram-negative bacteria that ferment the lactose produce acid which lowers the pH. This encourages dye absorption by the colonies and turns the colonies dark purple as the acid acts upon the dyes.

What bacteria grows on EMB plate?

Can fungi grow on EMB?

growing the molds or bacteria, different kind of media can be used. Some fungi can grow on EMB (e.g. http://jcm.asm.org/content/40/11/4281.short ).