What is a PHA blood test?

The Personal Health Assessment (PHA) is a complete health screening tool that identifies risk factors such as high blood pressure, high cholesterol, diabetes, obesity and much more. Being aware of your current health status is the first step toward a happier and healthier lifestyle.

What is Phytohemagglutinin used for?

One of the mitogens, phytohemagglutinin (PHA), has been widely used for the purpose of mitotic stimulation to human lymphocytes, and several different types of PHA, such as PHA-P, M, W and others were compared on their ability to induce mitoses and presented by other workers (5-10).

How do PHA and PWM differ from mitogens?

While PHA is a strong T-cell mitogen, PWM is a weak T-cell mitogen, but it induces B-cell activation and proliferation as well. This assay uses a method that directly measures the S-phase proliferation of lymphocytes through the use of Click chemistry.

What is Phytohaemagglutinin stimulation?

Stimulation of peripheral blood lymphocytes by phytohaemagglutinin presents a classic model of transition of cells from the quiescent G0 phase of the cell cycle into G1-, and subsequently progression through S-, G2- and M- phases of the cycle.

What is done during a PHA?

During the “virtual” portion of the PHA, the provider will conduct a review of the Soldier’s current medical conditions and self-reported health status as well as any new complaints the Soldier might have. The provider then makes recommendations for improvement of any identified health conditions.

How long does a PHA take?

Allow 10 business days for complete processing of the PHA and updating of all applicable databases. On occasion, a Soldier’s PHA will be referred to our office for review and profiling purposes. Allow for an additional 20 business days for processing by this office.

What is PHA immunology?

PHA is a non-specific stimulant of normal lymphocytes resulting in vitro in their blast-like transformation with increased RNA and DNA synthesis, mitosis, and the release of putative mediators of cell-mediated immunity.

How does PHA work on T cells?

Specifically, PHA binds to sugars on glycosylated surface proteins, including T cell receptor (TCR), and thereby crosslinks them. This triggers calcium-dependent signaling pathways leading to NFAT (nuclear factor of activated T cells) activation.

Does PHA activate B cells?

PHA does not activate human B cells, but it activates very well human T cells (see http://www.ncbi.nlm.nih.gov/pubmed/3263438). LPS actually DOES NOT activate human B cells. However, it can activate murine B cells that express TLR4 unlike human B cells.

What is a mitogen test for?

Test Includes: Viability of lymphocytes to help determine impaired T-cell function. Peripheral blood mononuclear cells (PBMC) are cultured in vitro with plant lectins (mitogens) such as phytohemagglutinin (PHA) and pokeweed mitogen (PWM).

How do I complete my PHA?

Soldiers can now complete their portion of the Periodic Health Assessment (PHA) online before their appointment with the medical provider. Complete your portion of the PHA, then schedule an appointment with your medical provider or MTF to complete the PHA process.

Do they drug test during a PHA?

Full Member But to answer the OP’s question – no, there is not a drug test associated with the USN PHA. But, expect to be randomly called to urinalysis as a command sponsored event and as a new check in to a new command.

What does the PHA consist of?

PHA is a four part assessment including a self-reported health assessment, record review, mental health review, and a face-to-face or person-to-person consultation with a certified PHA health care provider.

Does PHA stimulate monocytes?

Abstract. Human monocytes suppressed both the phytohaemagglutin (PHA) or antigen-induced lymphocyte proliferative response, when the monocyte: lymphocyte ratio was increased or when the monocytes were stimulated with zymosan or endotoxin.

What does PHA bind to?

How do you prepare PHA?

Add 100 μl PHA-L to each well in a a dilution series (10 dilutions, range 1 to 10 μg/ml). Incubate the mixture for 48 hours at +37°C. Add 0.5 μCi/well [3H]-thymidine. Continue the incubation for an additional 20 hours.

What does low mitogen mean?

A low response to Mitogen (<0.5 IU/mL) indicates an Indeterminate result when a blood sample. also has a negative response to the TB antigens. This pattern may occur with insufficient. lymphocytes, reduced lymphocyte activity due to prolonged specimen transport or improper.

What does mitogen nil mean?

• Mitogen-Nil The maximum non-specific stimulated interferon- gamma level that may be produced by the patient’s lymphocytes minus the Nil (i.e., basal) level of interferon-gamma.

How long does it take to get PHA results?

Within 48 hours of your PHA draw you will receive a text message indicating your results are available by logging in to the mobile app or the CareATC portal (careatc.com/patients) with the access code provided. Be sure to watch your personalized PHA Live video. Your results will arrive by U.S. mail within 2 weeks.