What does p62 stand for?

Also known as the ubiquitin-binding protein p62, it is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. By interacting with GATA4 and targeting it for degradation, it can inhibit GATA-4 associated senescence and senescence-associated secretory phenotype. SQSTM1.

Is p62 degraded in autophagy?

In the process, p62 is itself degraded and when autophagy is induced, a corresponding decrease in p62 levels is observed. Congruently, when autophagy is potently inhibited, i.e. genetic knock out of ATG core autophagy proteins like ATG5 or ATG7, p62 accumulates in the cell.

What is autophagy deficiency?

Autophagy deficiency leads to accumulation of ubiquitinated proteins, ER stress, and cell death in Arabidopsis.

What is p62 a marker of?

Labeling of p62 serves as a useful marker for the induction of autophagy, clearance of protein aggregates, and the inhibition of autophagy.

How is p62 degraded?

This protein functions as a critical receptor that identifies and binds to ubiquitinated proteins, delivering them via membrane-bound LC3 to the phagophore for subsequent degradation. Meanwhile, p62 itself is degraded by autolysosomes following the initiation of autophagy.

How do you learn autophagy flux?

Autophagosome flux can be quantified experimentally by completely blocking one of the steps in the pathway and measuring the initial rate of accumulation in the substrate for the inhibited step, such as, for example, the rate of autophagosome accumulation upon complete inhibition of the lysosomal fusion step.

How does vivo measure autophagy?

Measuring Autophagic Flux In Vivo Using Lysosomal Blockade A widely-used method consists of a pharmacological blockade acting either by interrupting the autophagosome-lysosome fusion step or by inhibiting lysosome-mediated enzymatic proteolysis [31].

What does autophagy failure effect?

Such a role of autophagy would mechanistically link effects on the prevention of tumor initiation, tumor progression, aging, and neurodegeneration. The precise mechanisms by which deficient autophagy compromises genomic stability are unclear.

What are the structural domains of FIP200?

Structural domains and binding proteins of FIP200. FIP200 consists of a putative nuclear localization signal (NLS) at N-terminus, a large coiled-coil (CC) domain and a leucine zipper (LZ) motif located at C-terminus. The proteins known to interact with specific regions of FIP200 are also shown.

Does FIP200 play a role in the regulation of cell differentiation?

The potential role of FIP200 in the regulation of cell differentiation was first implicated by its differential expression in human embryonic musculoskeletal cells [9]. FIP200 expresses at low level in immature embryonic musculoskeletal cells and its expression level increases concomitantly with the maturation of musculoskeletal cells [9].

Does FIP200 play a role in the development of heart and liver?

Therefore, a role of FIP200 in the regulation of cell size/cell growth may contribute to its potential function in heart and liver development as revealed in the current study. It has been shown that cell size increase is a prerequisite for cell proliferation during normal organ growth ( Conlon and Raff, 1999 ).

Is FIP200 a tumor suppressor gene?

Human FIP200 gene localizes in 8q11 chromosome, a region containing several loci of putative tumor suppressor genes, and loss of heterozygosity (LOH) of this region has been associated with various human cancers, including breast cancer [48], [49].