What color tubes are used for which tests?
What color tubes are used for which tests?
Albumin – Green, SST, or Plain Red.
What color tube is used for blood collection?
Yellow-top tube (ACD) This tube contains ACD, which is used for the collection of whole blood for special tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.
What is blood collection tubes?
Blood collection tubes containing heparin, which stabilizes the red blood cell membranes, are used for specialized hematology studies, such as red cell fragility tests and several specialized chemistry tests. Blood collection tubes containing sodium citrate are used for coagulation studies.
How are tubes with additives identified?
Each tube is topped with a color-coded plastic or rubber cap. Tubes often include additives that mix with the blood when collected, and the color of each tube’s plastic cap indicates which additives it contains.
What additives are in each tube?
Blood Collection Tubes
| Tube cap color | Additive |
|---|---|
| Red or gold (mottled or “tiger” top used with some tubes) | Serum tube with or without clot activator or gel |
| Green | Sodium or lithium heparin with or without gel |
| Lavender or pink | Potassium EDTA |
| Gray | Sodium fluoride, and sodium or potassium oxalate |
Which tube has no additive?
PHLEBOTOMY SERVICES
| STOPPER COLOR | CONTENTS | VOL. |
|---|---|---|
| Royal Blue | No additive (serum); special glass and stopper material | 7.0 mL |
| Red | No additive | 7.0 mL |
| Light Blue | 3.2% Sodium Citrate | 4.5 mL |
| Gold Top (Serum Separator, “SST”) | Contains separating gel and clot activator | 6.0 mL |
Why is EDTA used in blood collection?
It inhibits clotting by removing or chelating calcium from the blood. EDTA most important advantage is that it does not distort blood cells, making it ideal for the most hematological tests. It is known to cause erroneous results of platelet counts by automated hematological analysers yielding low platelets counts.
What additive is in the yellow tube?
PHLEBOTOMY SERVICES
| STOPPER COLOR | CONTENTS | USES/COMMENTS |
|---|---|---|
| Green | Sodium heparin(100 USP Units) | Ammonia, Lactate, HLA Typing |
| Tan | K2 EDTA | Lead levels |
| Yellow | ACD Solution A consists of trisodium citrate, citric acid and dextrose | DNA Studies, HIV Cultures |
| Pink | (K2)EDTA | Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load |
What is EDTA tube used for?
It’s a tube coated with spray-dried K2EDTA on its inner wall. EDTA acts as an anticoagulant, binding the calcium ions and interrupting the clotting of the blood sample. EDTA is used for most hematology procedures, assessing complete blood count, preparing EDTA plasma, whole blood collection and bone marrow specimens.
What additive is needed for CBC?
COLLECTION TUBES FOR PHLEBOTOMY
| Purple Top | |
|---|---|
| ADDITIVE | EDTA |
| MODE OF ACTION | Forms calcium salts to remove calcium |
| USES | Hematology (CBC) and Blood Bank (Crossmatch); requires full draw – invert 8 times to prevent clotting and platelet clumping |
Does a red top tube have an additive?
Red – Tube contains no additive, used for serum chemistry testing, serology, and blood bank testing.
What is the additive in a red-top tube?
What additive is in a GREY top tube?
Gray-top tube: Contains sodium fluoride (a preservative) and potassium oxalate (an anticoagulant). Use: Sodium fluoride whole blood or plasma.
What chemical is in a blue top tube?
2. Blue Top – Tube contains Sodium Citrate. Most often used for coagulation studies. Must be drawn prior to collection of serum tubes to prevent contamination with clot activator and interference with coagulation cascade.
Which tube additive preserves glucose?
Additive: All top tubes contain a glucose preservative (antiglycolytic agent): sodium fluoride (preserves glucose for 3 days) or lithium iodoacetate (preserves glucose for 24 hours). May also contain the anticoagulant potassium oxalate (prevents clotting by binding calcium).
What additive is in a SST tube?
PHLEBOTOMY SERVICES
| STOPPER COLOR | CONTENTS | VOL. |
|---|---|---|
| Red | No additive | 7.0 mL |
| Light Blue | 3.2% Sodium Citrate | 4.5 mL |
| Gold Top (Serum Separator, “SST”) | Contains separating gel and clot activator | 6.0 mL |
| Light Blue – Yellow Label on Tube | Thrombin | 2.0 mL |
What additive is in the pink tube?
Blood Collection Tubes
| Tube cap color | Additive | Function of Additive |
|---|---|---|
| Lavender or pink | Potassium EDTA | Prevents clotting by binding calcium |
| Gray | Sodium fluoride, and sodium or potassium oxalate | Fluoride inhibits glycolysis, and oxalate prevents clotting by precipitating calcium. |
What additive is in the green tube?
Lithium Heparin
Green – Tube contains Lithium Heparin, PST™ Gel additive with or without Amber Coating to protect light sensitive assays, used for plasma chemistry testing. Mix by inverting 10 – 15 times immediately after collection. Tube inversions prevent clotting.
What is difference between EDTA and heparin?
EDTA is most often used when you want to do hematological tests (red and white blood cell count, Hb, blood group), citrate for example damages cells so won’t we useful. Heparin is used for clinical chemistry tests such as cholesterol, CRP, hormones etc. It interferes with PCR, so if you want to do that use EDTA.
What additive is in red top tube?
Is EDTA a blood preservative?
Historically, EDTA has been recommended as the anticoagulant of choice for hematological testing because it allows the best preservation of cellular components and morphology of blood cells.
What preservatives are used in blood?
For several decades the standard blood preservative solution consisted of citrate, dextrose, and, later, phosphate (ACD and CPD). In 1978 a new solution containing adenine (CPDA-1) was introduced to permit extension of red cell shelf life from 21 to 35 days.
What are EDTA tubes?
What is the purpose of additives in blood collection tubes?
Additive: anticoagulant SPS (Sodium Polyanetholsulfonate)&ACD (acid citrate dextrose)
Which blood collection tubes should I use?
Collection Tubes (continued) The tubes used for CSF aspiration are generally pre-numbered 1 through 3 or 4, with tube 1 being the first tube filled. If possible, tube 1 should be reserved for non-routine studies. Tube 2 can be used for immunology and chemistry testing. Tube 3 can be used for microbiology testing.
What do additives do blood culture bottles contain?
The additives may include anticoagulants (EDTA, sodium citrate, heparin) or a gel with density between those of blood cells and blood plasma. Additionally, some tubes contain additives that preserve certain components of or substances within the blood, such as glucose.
What additive is in a gold top tube?
What is in a gold top tube? Gold-top serum separator tube (SST) This tube contains a clot activator and serum gel separator – used for various chemistry, serology, and immunology tests. Click to see full answer. Regarding this, what additive is in a gold top tube? Additionally, what blood tube colors are for which test?