What is Effectene transfection reagent?
What is Effectene transfection reagent?
Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines.
What is superfect transfection?
Superfect Transfection Reagent is a new transfection. reagent based on activated dendrimer technology for. super-high transfection results. Superfect Reagent. provides significant advantages over many other.
What is SuPerfect?
SuPerfect® fertiliser provides a balance of key nutrients such as phosphorus, sulphur and calcium for superior pasture performance. Developed to combine the proven advantages of superphosphate with a high quality granule, SuPerfect is better to handle, blend and spread.
What is PEI Max?
Polyethylenimine “Max” (PEI MAX) is a powerful, trusted, and cost-effective reagent widely considered as a current gold standard for both in vitro and in vivo transfection. PEI MAX has a high density of protonatable amino groups, with amino nitrogen as every third atom.
Should I change medium after transfection?
A complete media change can be performed 5 – 24 hours after transfection for very sensitive cells. For most cells, we recommend the media be changed only at 48 hours post-transfection until protocol optimization requires this extra media change. A complete media change should be performed at 48 hours post-transfection.
What is the NPK of superphosphate?
Single superphosphate is 0:8.8:0:11. This means that in 100 kg, there is 8.8 kg phosphorus, 11 kg sulfur. There is no nitrogen or potassium.
Is PEI toxic to cells?
2), the PEI itself can exert significant cellular toxicity so that the IC50 for LPEI and BPEI respectively were 74 mg and 37 mg (Fig. 3). To evaluate transfection efficiency and safety of BPEI for gene delivery, Yoon et al.