What is basic principle of HPLC?

Principle of HPLC The specific intermolecular interactions between the molecules of a sample and the packing material define their time “on-column”. Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.

What are the basic components of HPLC?

Going into more detail, HPLC consists of a variety of components, including a solvent delivery pump, a degassing unit, a sample injector, a column oven, a detector, and a data processor.

What is HPLC in simple terms?

High-performance liquid chromatography (HPLC) is an analytical technique to separate, identify, and quantify components in a mixture. It is the single biggest chromatography technique essential to most laboratories worldwide.

What are the detectors used in HPLC?

Lesson 6: Detectors for HPLC

• UV, VIS, and PDA Detectors. Refractive-Index Detector.
• UV, VIS, and PDA Detectors.
• Refractive-Index Detector.
• Evaporative Light Scattering Detector.
• Multi-Angle Light Scattering Detector.
• Mass Spectrometer.
• Conductivity Detector.
• Fluorescence Detector.

What is SST in HPLC?

System suitability test (SST) is a test to determine the suitability and effectiveness of chromatographic system prior to use. The performance of any chromatographic system may continuously change during their regular use, which can affect the reliability of the analytical results.

How many detectors are there in HPLC?

They are of three types, i.e. fixed wavelength detectors, variable wavelength detectors and the diode array detectors.

How many columns are in HPLC?

There are four main types of column in HPLC are used.

What is RT and RRT in HPLC?

In high pressure liquid chromatography (HPLC), the compound is injected through a column of different sized beads. The amount of time it takes for the compound to pass through the column is the retention time (RT). The relative retention time (RRT) is the comparison of the RT of one compound to another.

Why is TFA used in HPLC?

TFA is widely used as a mobile phase additive in the HPLC separation of biological molecules, such as proteins and peptides, because it acts as an ion-pairing reagent and equilibrates quickly so that it can be used with gradient elution.